Determination of Serum Apoprotein E by Rocket Immunoelectrophoresis
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چکیده
منابع مشابه
Low-density-lipoprotein apoprotein B in plasma as measured by radial immunodiffusion and rocket immunoelectrophoresis.
Radial immunodiffusion (RID) and rocket immunoelectrophoresis (RIE) are compared with respect to determination of LDL-bound apo B in plasma. Isolated VLDL could not enter a 15 g/L agarose gel when either technique was used. However, in the presence of plasma proteins, migration of VLDL into agarose was enhanced. Only when plasma samples were kept frozen before the assay was plasma VLDL unable t...
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suchung mit 51Cr-EDTA im abfallenden Plasmaspiegel. Atompraxis 13,357-360 (1967). 5. Stacy, B. D., and Thorburn, G. D., Chromiuin-51-ethylenediaminetetraacetate for the estimation of gbomerular filtration rate. Science 152, 1076-1077 (1966). 6. Watkin, D. M., Barrows, C. H., Jr., and Chow, B. F., Renal clearance of intravenously administered vitamin B12.Proc. Soc. Exp. Biol. Med. 107, 219-221 (...
متن کاملRocket and Two Dimensional Immunoelectrophoresis in Diagnosis of Caprine Brucellosis
BACKGROUND Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. METHODS Sera from 15 sheep, without any histo...
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We developed a rapid, accurate method for phenotyping apoprotein E that can be used for large-scale population studies. In this method, adapted from the method of Kamboh et al. (J Lipid Res 1988;29:1535-43), 10-microL plasma samples are incubated with dithiothreitol and Tween-20 for 15 min and then applied to 5% polyacrylamide gels containing ampholyte (pH 4.5-8) and urea (3 mol/L). After 2 h o...
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Serum concentrations for the cholesterol and triglyceride content of very low density lipoprotein (VLDL) and the cholesterol content of intermediate density lipoprotein (IDL), low density lipoprotein (LDL), and the high density lipoprotein fractions (HDL2), HDL3) were measured in 337 men and women with the apo E phenotype who were born in the Grampian Region of North East Scotland. The subjects...
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ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 1984
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.12.1_47